› 6hxu

X-ray diffraction
1.19Å resolution

Crystal structure of Human RHOB Q63L in complex with GTP

Released:
DOI: 10.2210/pdb6hxu/pdb
PDB entry 6hxu coloured by chain, front view.
PDB entry 6hxu coloured by chain, side view.
PDB entry 6hxu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation.
Bery N, Keller L, Soulié M, Gence R, Iscache AL, Cherier J, Cabantous S, Sordet O, Lajoie-Mazenc I, Pedelacq JD, Favre G, Olichon A
Cell Chem Biol 26 1544-1558.e6 (2019)
PMID: 31522999

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-related GTP-binding protein RhoB Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62745 (Residues: 1-183; Coverage: 93%)
Gene names: ARH6, ARHB, RHOB
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 38.89Å b: 61.46Å c: 76.69Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Exploring cellular biochemistry with nanobodies.
Cheloha et al. (2020)
10 more