› 6i2i

Electron Microscopy
3.6Å resolution

Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)

Released:
DOI: 10.2210/pdb6i2i/pdb
PDB entry 6i2i coloured by chain, front view.
PDB entry 6i2i coloured by chain, side view.
PDB entry 6i2i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
Adib R, Montgomery JM, Atherton J, O'Regan L, Richards MW, Straatman KR, Roth D, Straube A, Bayliss R, Moores CA, Fry AM
Sci Signal 12 (2019)
PMID: 31409757
Related structures: EMD-0331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139588 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68363 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 444 amino acids
Theoretical weight: 49.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07437 (Residues: 1-444; Coverage: 100%)
Gene names: OK/SW-cl.56, TUBB, TUBB5
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 2 x MG
Ligand G2P 1 x G2P
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0331

Quick links

Citations

6 review citations

Physiological and Pathological Roles of Mammalian NEK7.
Sun et al. (2020)
5 more

5 mentions without citation

Conformational changes in tubulin upon binding cryptophycin-52 reveal its mechanism of action.
Eren et al. (2021)
4 more