› 6i4x

X-ray diffraction
2.69Å resolution

Crystal structure of SOCS2:Elongin C:Elongin B in complex with erythropoietin receptor peptide

Released:
DOI: 10.2210/pdb6i4x/pdb
PDB entry 6i4x coloured by chain, front view.
PDB entry 6i4x coloured by chain, side view.
PDB entry 6i4x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into substrate recognition by the SOCS2 E3 ubiquitin ligase.
Kung WW, Ramachandran S, Makukhin N, Bruno E, Ciulli A
Nat Commun 10 2534 (2019)
PMID: 31182716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-126987 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 11.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-104; Coverage: 88%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Suppressor of cytokine signaling 2 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14508 (Residues: 30-198; Coverage: 85%)
Gene names: CIS2, SOCS2, SSI2, STATI2
Sequence domains:
Structure domains: SOCS box
Erythropoietin receptor Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.32 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
2 modified residues:
Ligand CAS 2 x CAS
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Unit cell:
a: 41.287Å b: 56.327Å c: 203.392Å
α: 90° β: 91.53° γ: 90°
R-values:
R R work R free
0.199 0.196 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

NEDD8 and ubiquitin ligation by cullin-RING E3 ligases.
Baek et al. (2021)
5 more

5 mentions without citation

Recent advances in synthetic and medicinal chemistry of phosphotyrosine and phosphonate-based phosphotyrosine analogues.
Makukhin et al. (2020)
4 more