› 6i5c

X-ray diffraction
2.95Å resolution

Long wavelength native-SAD phasing of Tubulin-Stathmin-TTL complex

Released:
DOI: 10.2210/pdb6i5c/pdb
Model geometry
Fit model/data
PDB entry 6i5c coloured by chain, front view.
PDB entry 6i5c coloured by chain, side view.
PDB entry 6i5c coloured by chain, top view.
Source organisms:
Primary publication:
Long-wavelength native-SAD phasing: opportunities and challenges.
Basu S, Olieric V, Leonarski F, Matsugaki N, Kawano Y, Takashi T, Huang CY, Yamada Y, Vera L, Olieric N, Basquin J, Wojdyla JA, Bunk O, Diederichs K, Yamamoto M, Wang M
OpenAccess logo IUCrJ 6 373-386 (2019)
PMID: 31098019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-237168 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 440 amino acids
Theoretical weight: 48.97 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P81947 (Residues: 1-440; Coverage: 98%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 431 amino acids
Theoretical weight: 48.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6B856 (Residues: 1-431; Coverage: 97%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
TUBULIN-TYROSINE LIGASE Chain: F

Ligands and Environments

7 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 3 x MG
Ligand CA 5 x CA
Ligand CL 2 x CL
Ligand GDP 2 x GDP
Ligand MES 2 x MES
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 104.24Å b: 156.83Å c: 179.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.208
Expression system: Escherichia coli

Quick links

Citations

15 citation in other articles

A shared vision for macromolecular crystallography over the next five years.
Förster et al. (2019)
14 more

1 mention without citation

Long-wavelength native-SAD phasing: opportunities and challenges.
Basu et al. (2019)