› 6i5m

X-ray diffraction
2.4Å resolution

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion

Released:
DOI: 10.2210/pdb6i5m/pdb
PDB entry 6i5m coloured by chain, front view.
PDB entry 6i5m coloured by chain, side view.
PDB entry 6i5m coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
The third structural switch in the archaeal translation initiation factor 2 (aIF2) molecule and its possible role in the initiation of GTP hydrolysis and the removal of aIF2 from the ribosome.
Nikonov O, Kravchenko O, Nevskaya N, Stolboushkina E, Garber M, Nikonov S
Acta Crystallogr D Struct Biol 75 392-399 (2019)
PMID: 30988256

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor 2 subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 45.85 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980A5 (Residues: 1-415; Coverage: 100%)
Gene names: SSO0412, eif2g
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand FMT 17 x FMT
Ligand MG 2 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R32
Unit cell:
a: 142.143Å b: 142.143Å c: 218.587Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.224
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

DAPL1 deficiency in mice impairs antioxidant defenses in the RPE and leads to retinal degeneration with AMD-like features.
Ma et al. (2023)