› 6iek

X-ray diffraction
2.7Å resolution

Structure of RVFV Gn and human monoclonal antibody R12

Released:
DOI: 10.2210/pdb6iek/pdb
PDB entry 6iek coloured by chain, front view.
PDB entry 6iek coloured by chain, side view.
PDB entry 6iek coloured by chain, top view.
Source organisms:
Primary publication:
Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus.
Wang Q, Ma T, Wu Y, Chen Z, Zeng H, Tong Z, Gao F, Qi J, Zhao Z, Chai Y, Yang H, Wong G, Bi Y, Wu L, Shi R, Yang M, Song J, Jiang H, An Z, Wang J, Yilma TD, Shi Y, Liu WJ, Liang M, Qin C, Gao GF, Yan J
Nat Microbiol 4 1231-1241 (2019)
PMID: 30936489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208966 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycoprotein N Chains: A, D
Molecule details ›
Chains: A, D
Length: 316 amino acids
Theoretical weight: 34.94 KDa
Source organism: Rift Valley fever virus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P03518 (Residues: 154-469; Coverage: 27%)
Gene name: GP
Sequence domains: Phlebovirus glycoprotein G1
Heavy chain of Fab R12 Chains: B, E
Molecule details ›
Chains: B, E
Length: 226 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins
Light chain of Fab R12 Chains: C, F
Molecule details ›
Chains: C, F
Length: 219 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 97.756Å b: 108.07Å c: 107.418Å
α: 90° β: 90.34° γ: 90°
R-values:
R R work R free
0.227 0.225 0.258
Expression systems:
  • Insect cell expression vector pTIE1
  • Mammalia

Quick links

Citations

6 review citations

Rift Valley fever: biology and epidemiology.
Wright et al. (2019)
5 more

1 mention without citation

Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality.
Goike et al. (2021)