› 6ii6

X-ray diffraction
2.1Å resolution

Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3)

Released:
DOI: 10.2210/pdb6ii6/pdb
PDB entry 6ii6 coloured by chain, front view.
PDB entry 6ii6 coloured by chain, side view.
PDB entry 6ii6 coloured by chain, top view.
Source organisms:
Primary publication:
Makes caterpillars floppy-like effector-containing MARTX toxins require host ADP-ribosylation factor (ARF) proteins for systemic pathogenicity.
Lee Y, Kim BS, Choi S, Lee EY, Park S, Hwang J, Kwon Y, Hyun J, Lee C, Kim JF, Eom SH, Kim MH
Proc Natl Acad Sci U S A 116 18031-18040 (2019)
PMID: 31427506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123287 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidase C80 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 40.82 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1CLG9 (Residues: 1313-1680; Coverage: 20%)
Gene name: rtxA1
Sequence domains: Yersinia/Haemophilus virulence surface antigen
ADP-ribosylation factor 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 172 amino acids
Theoretical weight: 19.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61204 (Residues: 13-176; Coverage: 91%)
Gene name: ARF3
Sequence domains: ADP-ribosylation factor family

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 53.293Å b: 117.523Å c: 186.348Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.232
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacterial Toxin and Effector Regulation of Intestinal Immune Signaling.
Woida et al. (2022)
1 more

6 mentions without citation

Cross-Kingdom Activation of Vibrio Toxins by ADP-Ribosylation Factor Family GTPases.
Herrera et al. (2020)
5 more