› 6ilx

X-ray diffraction
1.45Å resolution

Crystal structure of PETase W159F mutant from Ideonella sakaiensis

Released:
DOI: 10.2210/pdb6ilx/pdb
PDB entry 6ilx coloured by chain, front view.
PDB entry 6ilx coloured by chain, side view.
PDB entry 6ilx coloured by chain, top view.
Source organism: Ideonella sakaiensis
Primary publication:
Structural and functional characterization of polyethylene terephthalate hydrolase from Ideonella sakaiensis.
Liu C, Shi C, Zhu S, Wei R, Yin CC
Biochem Biophys Res Commun 508 289-294 (2019)
PMID: 30502092

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.5 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 28-290; Coverage: 100%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 51.087Å b: 51.077Å c: 85.378Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.195
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives.
Carr et al. (2020)
7 more

3 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
2 more