› 6irb

X-ray diffraction
2.66Å resolution

C-terminal coiled coil domain of Drosophila phospholipase C beta NORPA, selenomethionine

Released:
DOI: 10.2210/pdb6irb/pdb
PDB entry 6irb coloured by chain, front view.
PDB entry 6irb coloured by chain, side view.
PDB entry 6irb coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
An unexpected INAD PDZ tandem-mediated plcβ binding in Drosophila photo receptors.
Ye F, Huang Y, Li J, Ma Y, Xie C, Liu Z, Deng X, Wan J, Xue T, Liu W, Zhang M
Elife 7 (2018)
PMID: 30526850

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 25.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13217 (Residues: 863-1074; Coverage: 19%)
Gene names: CG3620, PLC-beta, norpA
Sequence domains: Protein of unknown function (DUF1154)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 36.958Å b: 37.591Å c: 140.13Å
α: 90.72° β: 89.86° γ: 119.44°
R-values:
R R work R free
0.228 0.225 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Cryptic binding properties of a transient folding intermediate in a PDZ tandem repeat.
Malagrinò et al. (2022)
3 more

1 mention without citation

An unexpected INAD PDZ tandem-mediated plcβ binding in Drosophila photo receptors.
Ye et al. (2018)