› 6j2j

X-ray diffraction
2.5Å resolution

Crystal structure of bat (Pteropus Alecto) MHC class I Ptal-N*01:01 in complex with MERS-CoV-derived peptide MERS-CoV-S3

Released:
DOI: 10.2210/pdb6j2j/pdb
PDB entry 6j2j coloured by chain, front view.
PDB entry 6j2j coloured by chain, side view.
PDB entry 6j2j coloured by chain, top view.
Source organisms:
Primary publication:
Peptide presentation by bat MHC class I provides new insight into the antiviral immunity of bats.
Lu D, Liu K, Zhang D, Yue C, Lu Q, Cheng H, Wang L, Chai Y, Qi J, Wang LF, Gao GF, Liu WJ
PLoS Biol 17 e3000436 (2019)
PMID: 31498797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-102855 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 277 amino acids
Theoretical weight: 32.12 KDa
Source organism: Pteropus alecto
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A125R585 (Residues: 25-301; Coverage: 81%)
Gene name: Ptal-N
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Spike protein S1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 997 Da
Source organism: Middle East respiratory syndrome-related coronavirus
Expression system: Not provided
UniProt:
  • Canonical: K9N5Q8 (Residues: 422-430; Coverage: 1%)
Gene names: 3, S

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 101.035Å b: 102.714Å c: 177.674Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.267
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

3 review citations

Exploring the Role of Innate Lymphocytes in the Immune System of Bats and Virus-Host Interactions.
Sia et al. (2022)
2 more

1 mention without citation

Peptide presentation by bat MHC class I provides new insight into the antiviral immunity of bats.
Lu et al. (2019)