› 6j8y

X-ray diffraction
2.4Å resolution

Crystal structure of the human RAD9-HUS1-RAD1-RHINO complex

Released:
DOI: 10.2210/pdb6j8y/pdb
PDB entry 6j8y coloured by chain, front view.
PDB entry 6j8y coloured by chain, side view.
PDB entry 6j8y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp.
Hara K, Iida N, Tamafune R, Ohashi E, Sakurai H, Ishikawa Y, Hishiki A, Hashimoto H
J Biol Chem 295 899-904 (2020)
PMID: 31776186

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130057 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cell cycle checkpoint control protein RAD9A Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99638 (Residues: 1-270; Coverage: 69%)
Gene name: RAD9A
Sequence domains: Rad9
Checkpoint protein HUS1 Chain: B
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 32.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60921 (Residues: 2-280; Coverage: 100%)
Gene name: HUS1
Sequence domains: Hus1-like protein
Cell cycle checkpoint protein RAD1 Chain: C
Molecule details ›
Chain: C
Length: 282 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60671 (Residues: 1-282; Coverage: 100%)
Gene names: RAD1, REC1
Sequence domains: Repair protein Rad1/Rec1/Rad17
RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 Chain: D
Molecule details ›
Chain: D
Length: 20 amino acids
Theoretical weight: 2.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BSD3 (Residues: 45-64; Coverage: 8%)
Gene names: C12orf32, HKMT1188, RHINO, RHNO1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 52.937Å b: 136.19Å c: 154.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.258
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Phosphorylation-dependent assembly of DNA damage response systems and the central roles of TOPBP1.
Day et al. (2021)
2 other articles

2 mentions without citation

Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp.
Hara et al. (2020)
1 more