› 6jo5

Electron Microscopy
2.9Å resolution

Structure of the green algal photosystem I supercomplex with light-harvesting complex I

Released:
DOI: 10.2210/pdb6jo5/pdb
PDB entry 6jo5 coloured by chain, front view.
PDB entry 6jo5 coloured by chain, side view.
PDB entry 6jo5 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of the green algal photosystem I supercomplex with a decameric light-harvesting complex I.
Suga M, Ozawa SI, Yoshida-Motomura K, Akita F, Miyazaki N, Takahashi Y
Nat Plants 5 626-636 (2019)
PMID: 31182847
Related structures: EMD-9853

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108667 (preferred)
Entry contents:
20 distinct polypeptide molecules
Macromolecules (20 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 751 amino acids
Theoretical weight: 83.24 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P12154 (Residues: 1-751; Coverage: 100%)
Gene names: ps1a1, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 755 amino acids
Theoretical weight: 84.95 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P09144 (Residues: 4-735; Coverage: 100%)
Gene names: ps1a2, psaB
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 81 amino acids
Theoretical weight: 8.87 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q00914 (Residues: 1-81; Coverage: 100%)
Gene name: psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit II, chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 161 amino acids
Theoretical weight: 17.73 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q39615 (Residues: 36-196; Coverage: 82%)
Gene name: psaD
Sequence domains: PsaD
Photosystem I reaction center subunit IV, chloroplastic Chain: E
Molecule details ›
Chain: E
Length: 73 amino acids
Theoretical weight: 8.14 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P12352 (Residues: 25-97; Coverage: 75%)
Gene name: PSAE
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III, chloroplastic Chain: F
Molecule details ›
Chain: F
Length: 165 amino acids
Theoretical weight: 17.96 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P12356 (Residues: 63-227; Coverage: 73%)
Gene name: PSAF
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit V, chloroplastic Chain: G
Molecule details ›
Chain: G
Length: 94 amino acids
Theoretical weight: 9.83 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P14224 (Residues: 31-124; Coverage: 75%)
Gene name: PSAG
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 106 amino acids
Theoretical weight: 10.59 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IFG7 (Residues: 1-106; Coverage: 100%)
Gene name: CHLRE_03g165100v5
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 41 amino acids
Theoretical weight: 4.75 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P59777 (Residues: 1-41; Coverage: 100%)
Gene name: psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Photosystem I reaction center subunit psaK, chloroplastic Chain: K
Molecule details ›
Chain: K
Length: 87 amino acids
Theoretical weight: 8.43 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P14225 (Residues: 27-113; Coverage: 77%)
Gene name: PSAK
Sequence domains: Photosystem I psaG / psaK
PSI subunit V Chain: L
Molecule details ›
Chain: L
Length: 156 amino acids
Theoretical weight: 16.12 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IL32 (Residues: 41-196; Coverage: 80%)
Gene name: CHLRE_12g486300v5
Sequence domains: Photosystem I reaction centre subunit XI
Chlorophyll a-b binding protein, chloroplastic Chains: 1, Z
Molecule details ›
Chains: 1, Z
Length: 194 amino acids
Theoretical weight: 20.37 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q05093 (Residues: 35-228; Coverage: 85%)
Gene names: CHLRE_06g283050v5, LhcI-6, Lhca1
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 3
Molecule details ›
Chain: 3
Length: 268 amino acids
Theoretical weight: 29.44 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
Chlorophyll a-b binding protein, chloroplastic Chain: 7
Molecule details ›
Chain: 7
Length: 215 amino acids
Theoretical weight: 23.45 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q84Y02 (Residues: 27-241; Coverage: 89%)
Gene names: CHLRE_16g687900v5, Lhca
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 8
Molecule details ›
Chain: 8
Length: 217 amino acids
Theoretical weight: 23.29 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q75VY7 (Residues: 27-243; Coverage: 89%)
Gene names: CHLRE_06g272650v5, LhcI-4
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 4
Molecule details ›
Chain: 4
Length: 236 amino acids
Theoretical weight: 25.91 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q75VZ0 (Residues: 29-264; Coverage: 89%)
Gene names: CHLRE_10g452050v5, LhcI-1
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 5
Molecule details ›
Chain: 5
Length: 229 amino acids
Theoretical weight: 25.26 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q75VY8 (Residues: 29-257; Coverage: 89%)
Gene names: CHLRE_10g425900v5, LhcI-3
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 6
Molecule details ›
Chain: 6
Length: 232 amino acids
Theoretical weight: 25.18 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: Q75VY6 (Residues: 26-257; Coverage: 90%)
Gene names: CHLRE_06g278213v5, LhcI-5
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 2
Molecule details ›
Chain: 2
Length: 221 amino acids
Theoretical weight: 24.33 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8IKC8 (Residues: 26-246; Coverage: 90%)
Gene name: CHLRE_12g508750v5
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 9
Molecule details ›
Chain: 9
Length: 189 amino acids
Theoretical weight: 20.37 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Chlamydomonas reinhardtii
UniProt:
  • Canonical: A8ITV3 (Residues: 25-213; Coverage: 89%)
Gene name: CHLRE_07g344950v5
Sequence domains: Chlorophyll A-B binding protein

Ligands and Environments

10 bound ligands:
Ligand CL0 1 x CL0
Ligand CLA 218 x CLA
Ligand PQN 2 x PQN
Ligand LHG 12 x LHG
Ligand BCR 32 x BCR
Ligand SF4 3 x SF4
Ligand DGD 1 x DGD
Ligand LMG 2 x LMG
Ligand CHL 20 x CHL
Ligand LUT 22 x LUT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-9853
Expression system: Chlamydomonas reinhardtii

Quick links

Citations

5 review citations

Beyond 'seeing is believing': the antenna size of the photosystems in vivo.
Croce R. (2020)
4 more

8 mentions without citation

Opportunities and challenges for assigning cofactors in cryo-EM density maps of chlorophyll-containing proteins.
Gisriel et al. (2020)
7 more