› 6jtt

X-ray diffraction
2.51Å resolution

MHETase in complex with BHET

Released:
DOI: 10.2210/pdb6jtt/pdb
PDB entry 6jtt coloured by chain, front view.
PDB entry 6jtt coloured by chain, side view.
PDB entry 6jtt coloured by chain, top view.
Source organism: Ideonella sakaiensis
Primary publication:
Decomposition of PET film by MHETase using Exo-PETase function
Sagong H-Y, Seo H, Kim T, Son H, Joo S, Lee S, Kim S, Woo J-S, Hwang S, Kim K-J
ACS Catalysis 10 4805-4812 (2020)

Function and Biology Details

Reaction catalysed: 
Ethylene terephthalate + H(2)O = terephthalate + ethylene glycol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mono(2-hydroxyethyl) terephthalate hydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 621 amino acids
Theoretical weight: 65.25 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K8P8E7 (Residues: 17-603; Coverage: 100%)
Gene name: ISF6_0224
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

3 bound ligands:
Ligand C9C 2 x C9C
Ligand CA 3 x CA
Ligand C8X 1 x C8X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 215.259Å b: 173.799Å c: 106.671Å
α: 90° β: 119.51° γ: 90°
R-values:
R R work R free
0.192 0.19 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links