› 6k0x

X-ray diffraction
2.2Å resolution

Structure of N6AMT1-TRMT112 Complex with SAM

Released:
DOI: 10.2210/pdb6k0x/pdb
PDB entry 6k0x coloured by chain, front view.
PDB entry 6k0x coloured by chain, side view.
PDB entry 6k0x coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Guo Q, Liao S, Xu C, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193774 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyltransferase N6AMT1 Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9Y5N5 (Residues: 2-214; Coverage: 100%)
Gene names: C21orf127, HEMK2, KMT9, N6AMT1, PRED28
Sequence domains: Methyltransferase small domain
Multifunctional methyltransferase subunit TRM112-like protein Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9UI30 (Residues: 1-125; Coverage: 100%)
Gene names: AD-001, HSPC152, HSPC170, TRMT112
Sequence domains: Trm112p-like protein

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P6122
Unit cell:
a: 109.607Å b: 109.607Å c: 130.368Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.214
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

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