› 6k42

Electron Microscopy
4.1Å resolution

cryo-EM structure of alpha2BAR-Gi1 complex

Released:
DOI: 10.2210/pdb6k42/pdb
PDB entry 6k42 coloured by chain, front view.
PDB entry 6k42 coloured by chain, side view.
PDB entry 6k42 coloured by chain, top view.
Source organisms:
Primary publication:
Activation of the α2B adrenoceptor by the sedative sympatholytic dexmedetomidine.
Yuan D, Liu Z, Kaindl J, Maeda S, Zhao J, Sun X, Xu J, Gmeiner P, Wang HW, Kobilka BK
Nat Chem Biol 16 507-512 (2020)
PMID: 32152538
Related structures: EMD-9912

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209398 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera
UniProt:
  • Canonical: P63097 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Mus musculus
Expression system: Spodoptera
UniProt:
  • Canonical: P62874 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Alpha-2A adrenergic receptor; Alpha-2B adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 512 amino acids
Theoretical weight: 58.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera
UniProt:
  • Canonical: P08913 (Residues: 37-44; Coverage: 2%)
  • Canonical: P18089 (Residues: 9-450; Coverage: 68%)
Gene names: ADRA2A, ADRA2B, ADRA2L1, ADRA2R, ADRA2RL1, ADRAR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFv Chain: H
Molecule details ›
Chain: H
Length: 307 amino acids
Theoretical weight: 32.9 KDa
Source organism: Mus musculus
Expression system: Spodoptera

Ligands and Environments

1 bound ligand:
Ligand CZX 1 x CZX
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-9912
Expression system: Spodoptera

Quick links

Citations

6 review citations

Ligands of Adrenergic Receptors: A Structural Point of View.
Wu et al. (2021)
5 more

6 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
5 more