› 6k67

X-ray diffraction
1.95Å resolution

Application of anti-helix antibodies in protein structure determination (9011-3LRH)

Released:
DOI: 10.2210/pdb6k67/pdb
PDB entry 6k67 coloured by chain, front view.
PDB entry 6k67 coloured by chain, side view.
PDB entry 6k67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Application of antihelix antibodies in protein structure determination.
Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO
Proc Natl Acad Sci U S A 116 17786-17791 (2019)
PMID: 31371498

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209622 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3LRH introbody Chains: A, C
Molecule details ›
Chains: A, C
Length: 135 amino acids
Theoretical weight: 14.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Calmodulin-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 104 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP25 (Residues: 9-81; Coverage: 49%)
Gene names: CALM3, CALML2, CAM3, CAMC, CAMIII
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: R3
Unit cell:
a: 115.298Å b: 115.298Å c: 93.275Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.236
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Mukherjee et al. (2020)
7 more

1 mention without citation

Application of antihelix antibodies in protein structure determination.
Kim et al. (2019)