› 6k71

Electron Microscopy
4.3Å resolution

eIF2 - eIF2B complex

Released:
DOI: 10.2210/pdb6k71/pdb
PDB entry 6k71 coloured by chain, front view.
PDB entry 6k71 coloured by chain, side view.
PDB entry 6k71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for eIF2B inhibition in integrated stress response.
Kashiwagi K, Yokoyama T, Nishimoto M, Takahashi M, Sakamoto A, Yonemochi M, Shirouzu M, Ito T
Science 364 495-499 (2019)
PMID: 31048492
Related structures: EMD-9840

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138585 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Translation initiation factor eIF2B subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 351 amino acids
Theoretical weight: 39.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 1-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Translation initiation factor eIF2B subunit delta Chains: G, H
Molecule details ›
Chains: G, H
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: I, J
Molecule details ›
Chains: I, J
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Eukaryotic translation initiation factor 2 subunit 1 Chain: K
Molecule details ›
Chain: K
Length: 315 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05198 (Residues: 1-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
Eukaryotic translation initiation factor 2 subunit 2 Chain: M
Molecule details ›
Chain: M
Length: 333 amino acids
Theoretical weight: 38.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20042 (Residues: 1-333; Coverage: 100%)
Gene names: EIF2B, EIF2S2
Sequence domains: Domain found in IF2B/IF5
Eukaryotic translation initiation factor 2 subunit 3 Chain: P
Molecule details ›
Chain: P
Length: 472 amino acids
Theoretical weight: 51.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-9840
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The integrated stress response: From mechanism to disease.
Costa-Mattioli et al. (2020)
13 more

2 mentions without citation

ISRIB Blunts the Integrated Stress Response by Allosterically Antagonising the Inhibitory Effect of Phosphorylated eIF2 on eIF2B.
Zyryanova et al. (2021)
1 more