› 6k7o

X-ray diffraction
3Å resolution

Complex structure of LILRB4 and h128-3 antibody

Released:
DOI: 10.2210/pdb6k7o/pdb
PDB entry 6k7o coloured by chain, front view.
PDB entry 6k7o coloured by chain, side view.
PDB entry 6k7o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development.
Gui X, Deng M, Song H, Chen Y, Xie J, Li Z, He L, Huang F, Xu Y, Anami Y, Yu H, Yu C, Li L, Yuan Z, Xu X, Wang Q, Chai Y, Huang T, Shi Y, Tsuchikama K, Liao XC, Xia N, Gao GF, Zhang N, Zhang CC, An Z
Cancer Immunol Res 7 1244-1257 (2019)
PMID: 31213474

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212630 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Leukocyte immunoglobulin-like receptor subfamily B member 4 Chains: A, P, Q, R
Molecule details ›
Chains: A, P, Q, R
Length: 95 amino acids
Theoretical weight: 10.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8NHJ6 (Residues: 24-118; Coverage: 22%)
Gene names: ILT3, LILRB4, LIR5
Sequence domains: Immunoglobulin domain
h128-3 Fab heavy chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 228 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
h128-3 Fab light chain Chains: C, E, G, L
Molecule details ›
Chains: C, E, G, L
Length: 218 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P62
Unit cell:
a: 187.276Å b: 187.276Å c: 183.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.27
Expression systems:
  • Escherichia coli BL21
  • Homo sapiens

Quick links

Citations

10 review citations

New strategies to treat AML: novel insights into AML survival pathways and combination therapies.
Nair et al. (2021)
9 more

1 mention without citation

Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development.
Gui et al. (2019)