› 6k8o

X-ray diffraction
2.5Å resolution

Crystal structure of the Sulfolobus solfataricus topoisomerase III in complex with DNA

Released:
DOI: 10.2210/pdb6k8o/pdb
PDB entry 6k8o coloured by chain, front view.
PDB entry 6k8o coloured by chain, side view.
PDB entry 6k8o coloured by chain, top view.
Source organisms:
Entry authors: Wang HQ, Zhang JH, Zheng X, Zheng ZF, Dong YH, Huang L, Gong Y

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120127 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA topoisomerase Chain: A
Molecule details ›
Chain: A
Length: 668 amino acids
Theoretical weight: 76.68 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZJ8 (Residues: 1-668; Coverage: 100%)
Gene names: SSO0907, topA
Sequence domains:
DNA (5'-D(*GP*CP*AP*AP*GP*GP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.45 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P42212
Unit cell:
a: 150.731Å b: 150.731Å c: 97.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.259
Expression systems:
  • Escherichia coli
  • Not provided

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