› 6kf7

X-ray diffraction
1.8Å resolution

Microbial Hormone-sensitive lipase E53 mutant S285G

Released:
DOI: 10.2210/pdb6kf7/pdb
PDB entry 6kf7 coloured by chain, front view.
PDB entry 6kf7 coloured by chain, side view.
PDB entry 6kf7 coloured by chain, top view.
Source organism: Erythrobacter longus
Entry authors: Xiaochen Y, Zhengyang L, Xuewei X, Jixi L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 33.13 KDa
Source organism: Erythrobacter longus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A074MDU6 (Residues: 1-314; Coverage: 100%)
Gene name: EH31_02760
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

7 bound ligands:
Ligand D8F 4 x D8F
Ligand SO4 2 x SO4
Ligand EDO 13 x EDO
Ligand GOL 16 x GOL
Ligand 6NA 2 x 6NA
Ligand PGE 1 x PGE
Ligand NPO 1 x NPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Unit cell:
a: 70.657Å b: 129.715Å c: 219.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.191
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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