› 6kly

X-ray diffraction
2.01Å resolution

Crystal structure of the type III effector XopAI from Xanthomonas axonopodis pv. citri in space group P43212

Released:
DOI: 10.2210/pdb6kly/pdb
PDB entry 6kly coloured by chain, front view.
PDB entry 6kly coloured by chain, side view.
PDB entry 6kly coloured by chain, top view.
Source organism: Xanthomonas citri
Primary publication:
Crystal Structure-Based Exploration of Arginine-Containing Peptide Binding in the ADP-Ribosyltransferase Domain of the Type III Effector XopAI Protein.
Liu JH, Yang JY, Hsu DW, Lai YH, Li YP, Tsai YR, Hou MH
Int J Mol Sci 20 (2019)
PMID: 31615004

Function and Biology Details

Reaction catalysed: 
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+)--protein-arginine ADP-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.87 KDa
Source organism: Xanthomonas citri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0U5GCU5 (Residues: 1-255; Coverage: 100%)
Gene name: XAC3562_710001
Sequence domains: NAD:arginine ADP-ribosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1, NSRRC BEAMLINE BL13B1
Spacegroup: P43212
Unit cell:
a: 73.05Å b: 73.05Å c: 114.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

What the Wild Things Do: Mechanisms of Plant Host Manipulation by Bacterial Type III-Secreted Effector Proteins.
Schreiber et al. (2021)
1 other article

2 mentions without citation

Crystal Structure-Based Exploration of Arginine-Containing Peptide Binding in the ADP-Ribosyltransferase Domain of the Type III Effector XopAI Protein.
Liu et al. (2019)
1 more