› 6ktr

X-ray diffraction
2.6Å resolution

Crystal structure of fibroblast growth factor 19 in complex with Fab

Released:
DOI: 10.2210/pdb6ktr/pdb
PDB entry 6ktr coloured by chain, front view.
PDB entry 6ktr coloured by chain, side view.
PDB entry 6ktr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel Abs targeting the N-terminus of fibroblast growth factor 19 inhibit hepatocellular carcinoma growth without bile-acid-related side-effects.
Liu H, Zheng S, Hou X, Liu X, Du K, Lv X, Li Y, Yang F, Li W, Sui J
Cancer Sci 111 1750-1760 (2020)
PMID: 32061104

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208187 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
G1A8-Fab-HC Chains: A, F
Molecule details ›
Chains: A, F
Length: 233 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
G1A8-Fab-LC Chains: B, G
Molecule details ›
Chains: B, G
Length: 216 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fibroblast growth factor 19 Chains: C, D
Molecule details ›
Chains: C, D
Length: 218 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O95750 (Residues: 23-216; Coverage: 100%)
Gene names: FGF19, UNQ334/PRO533
Sequence domains: Fibroblast growth factor

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 84.968Å b: 104.125Å c: 165.365Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.253
Expression system: Homo sapiens

Quick links

Citations

1 review citation

New developments in the biology of fibroblast growth factors.
Ornitz et al. (2022)
2 other articles

2 mentions without citation

An ultrapotent pan-β-coronavirus lineage B (β-CoV-B) neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope.
Liu et al. (2022)
1 more