› 6l6l

X-ray diffraction
2.78Å resolution

Structural basis of NR4A2 homodimers binding to selective Nur-responsive elements

Released:
DOI: 10.2210/pdb6l6l/pdb
PDB entry 6l6l coloured by chain, front view.
PDB entry 6l6l coloured by chain, side view.
PDB entry 6l6l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of binding of homodimers of the nuclear receptor NR4A2 to selective Nur-responsive DNA elements.
Jiang L, Dai S, Li J, Liang X, Qu L, Chen X, Guo M, Chen Z, Chen L, Wei H, Chen Y
J Biol Chem 294 19795-19803 (2019)
PMID: 31723028

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120187 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 4 group A member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43354 (Residues: 262-346; Coverage: 14%)
Gene names: NOT, NR4A2, NURR1, TINUR
Sequence domains: Zinc finger, C4 type (two domains)
DNA (5'-D(*AP*AP*AP*GP*GP*TP*CP*AP*AP*AP*CP*TP*GP*TP*GP*AP*CP*CP*TP*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DNA (5'-D(P*TP*AP*TP*AP*GP*GP*TP*CP*AP*CP*AP*GP*TP*TP*TP*GP*AP*CP*CP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 42.405Å b: 64.315Å c: 60.776Å
α: 90° β: 96.84° γ: 90°
R-values:
R R work R free
0.22 0.216 0.252
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Paradoxical Roles of Orphan Nuclear Receptor 4A (NR4A) in Cancer.
Safe et al. (2021)
2 more

2 mentions without citation

Structural basis of binding of homodimers of the nuclear receptor NR4A2 to selective Nur-responsive DNA elements.
Jiang et al. (2019)
1 more