› 6ldh

X-ray diffraction
2Å resolution

REFINED CRYSTAL STRUCTURE OF DOGFISH M4 APO-LACTATE DEHYDROGENASE

Released:
DOI: 10.2210/pdb6ldh/pdb
PDB entry 6ldh coloured by chain, front view.
PDB entry 6ldh coloured by chain, side view.
PDB entry 6ldh coloured by chain, top view.
Source organism: Squalus acanthias
Primary publication:
Refined crystal structure of dogfish M4 apo-lactate dehydrogenase.
Abad-Zapatero C, Griffith JP, Sussman JL, Rossmann MG
J Mol Biol 198 445-67 (1987)
PMID: 3430615

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.35 KDa
Source organism: Squalus acanthias
Expression system: Not provided
UniProt:
  • Canonical: P00341 (Residues: 2-333; Coverage: 99%)
Gene name: ldha
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Unit cell:
a: 146.8Å b: 146.8Å c: 155.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

Review: Protein function at thermal extremes: balancing stability and flexibility.
Fields PA. (2001)
8 more

12 mentions without citation

Hot spots in cold adaptation: localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes.
Fields et al. (1998)
11 more