› 6le5

X-ray diffraction
3.1Å resolution

Crystal structure of the mitochondrial calcium uptake 1 and 2 heterodimer (MICU1-MICU2 heterodimer) in an apo state

Released:
DOI: 10.2210/pdb6le5/pdb
PDB entry 6le5 coloured by chain, front view.
PDB entry 6le5 coloured by chain, side view.
PDB entry 6le5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MICU1-MICU2 heterodimer provides insights into the gatekeeping threshold shift.
Park J, Lee Y, Park T, Kang JY, Mun SA, Jin M, Yang J, Eom SH
IUCrJ 7 355-365 (2020)
PMID: 32148862

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184952 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium uptake protein 1, mitochondrial Chains: A, D, E, G
Molecule details ›
Chains: A, D, E, G
Length: 348 amino acids
Theoretical weight: 40.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BPX6 (Residues: 97-444; Coverage: 73%)
Gene names: CALC, CBARA1, MICU1
Sequence domains: EF hand
Calcium uptake protein 2, mitochondrial Chains: B, C, F, H
Molecule details ›
Chains: B, C, F, H
Length: 325 amino acids
Theoretical weight: 39.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IYU8 (Residues: 84-401; Coverage: 73%)
Gene names: EFHA1, MICU2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 62.97Å b: 173.72Å c: 148Å
α: 90° β: 93.88° γ: 90°
R-values:
R R work R free
0.295 0.293 0.332
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular nature and physiological role of the mitochondrial calcium uniporter channel.
Alevriadou et al. (2021)
5 more

3 mentions without citation

Structure of the MICU1-MICU2 heterodimer provides insights into the gatekeeping threshold shift.
Park et al. (2020)
2 more