› 6lgv

X-ray diffraction
1.85Å resolution

Crystal structure of a cysteine-pair mutant (P10C-S291C) of a bacterial bile acid transporter in an inward-facing state complexed with citrate

Released:
DOI: 10.2210/pdb6lgv/pdb
PDB entry 6lgv coloured by chain, front view.
PDB entry 6lgv coloured by chain, side view.
PDB entry 6lgv coloured by chain, top view.
Source organism: Yersinia frederiksenii
Primary publication:
Substrate binding in the bile acid transporter ASBTYf from Yersinia frederiksenii.
Wang X, Lyu Y, Ji Y, Sun Z, Zhou X
Acta Crystallogr D Struct Biol 77 117-125 (2021)
PMID: 33404531

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter, sodium/bile acid symporter family Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.39 KDa
Source organism: Yersinia frederiksenii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A380PV03 (Residues: 1-307; Coverage: 100%)
Gene name: NCTC11470_02445
Sequence domains: Sodium Bile acid symporter family

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand A6L 5 x A6L
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21212
Unit cell:
a: 71.798Å b: 91.779Å c: 44.855Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Multitasking Na+/Taurocholate Cotransporting Polypeptide (NTCP) as a Drug Target for HBV Infection: From Protein Engineering to Drug Discovery.
Zakrzewicz et al. (2022)
1 more

1 mention without citation

Structure Reveals Homology in Elevator Transporters.
Trebesch et al. (2023)