› 6lml

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the human glucagon receptor in complex with Gi1

Released:
DOI: 10.2210/pdb6lml/pdb
PDB entry 6lml coloured by chain, front view.
PDB entry 6lml coloured by chain, side view.
PDB entry 6lml coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Gs and Gi recognition by the human glucagon receptor.
Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B
Science 367 1346-1352 (2020)
PMID: 32193322
Related structures: EMD-0918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208387 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: D
Molecule details ›
Chain: D
Length: 247 amino acids
Theoretical weight: 26.34 KDa
Source organism: Mus musculus
Expression system: unidentified baculovirus
Glucagon Chain: E
Molecule details ›
Chain: E
Length: 29 amino acids
Theoretical weight: 3.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01275 (Residues: 53-81; Coverage: 18%)
Gene name: GCG
Sequence domains: Peptide hormone
Glucagon receptor Chain: R
Molecule details ›
Chain: R
Length: 422 amino acids
Theoretical weight: 48.57 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P47871 (Residues: 27-432; Coverage: 90%)
Gene name: GCGR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-0918
Expression systems:
  • unidentified baculovirus
  • Not provided

Quick links

Citations

12 review citations

Regulation of the JAK2-STAT5 Pathway by Signaling Molecules in the Mammary Gland.
Tian et al. (2020)
11 more

13 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
12 more