› 6lpb

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein

Released:
DOI: 10.2210/pdb6lpb/pdb
PDB entry 6lpb coloured by chain, front view.
PDB entry 6lpb coloured by chain, side view.
PDB entry 6lpb coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein.
Kobayashi K, Shihoya W, Nishizawa T, Kadji FMN, Aoki J, Inoue A, Nureki O
Nat Struct Mol Biol 27 274-280 (2020)
PMID: 32157248
Related structures: EMD-0940

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210739 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Pituitary adenylate cyclase-activating polypeptide 38 Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18509 (Residues: 132-169; Coverage: 25%)
Gene name: ADCYAP1
Sequence domains: Peptide hormone
Pituitary adenylate cyclase-activating polypeptide type I receptor Chain: R
Molecule details ›
Chain: R
Length: 403 amino acids
Theoretical weight: 46.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41586 (Residues: 21-417; Coverage: 89%)
Gene name: ADCYAP1R1
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 63%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-0940
Expression systems:
  • Not provided
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

6 review citations

Novel Analgesics with Peripheral Targets.
Ciotu et al. (2020)
5 more

8 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
7 more