› 6m1h

Electron Microscopy
3.6Å resolution

CryoEM structure of human PAC1 receptor in complex with maxadilan

Released:
DOI: 10.2210/pdb6m1h/pdb
PDB entry 6m1h coloured by chain, front view.
PDB entry 6m1h coloured by chain, side view.
PDB entry 6m1h coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism.
Wang J, Song X, Zhang D, Chen X, Li X, Sun Y, Li C, Song Y, Ding Y, Ren R, Harrington EH, Hu LA, Zhong W, Xu C, Huang X, Wang HW, Ma Y
Cell Res 30 436-445 (2020)
PMID: 32047270
Related structures: EMD-30047

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211011 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Pituitary adenylate cyclase-activating polypeptide type I receptor Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 47.97 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P41586 (Residues: 23-438; Coverage: 88%)
Gene name: ADCYAP1R1
Sequence domains:
Maxadilan Chain: B
Molecule details ›
Chain: B
Length: 61 amino acids
Theoretical weight: 6.88 KDa
Source organism: Lutzomyia longipalpis
Expression system: Not provided
UniProt:
  • Canonical: P30659 (Residues: 24-82; Coverage: 94%)
Nanobody 35 Chain: C
Molecule details ›
Chain: C
Length: 134 amino acids
Theoretical weight: 14.71 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: E
Molecule details ›
Chain: E
Length: 341 amino acids
Theoretical weight: 37.47 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: F
Molecule details ›
Chain: F
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-30047
Expression systems:
  • Insect BA phytoplasma
  • Not provided
  • Escherichia coli

Quick links

Citations

6 review citations

Novel Analgesics with Peripheral Targets.
Ciotu et al. (2020)
5 more

6 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
5 more