› 6m1i

Electron Microscopy
3.5Å resolution

CryoEM structure of human PAC1 receptor in complex with PACAP38

Released:
DOI: 10.2210/pdb6m1i/pdb
PDB entry 6m1i coloured by chain, front view.
PDB entry 6m1i coloured by chain, side view.
PDB entry 6m1i coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism.
Wang J, Song X, Zhang D, Chen X, Li X, Sun Y, Li C, Song Y, Ding Y, Ren R, Harrington EH, Hu LA, Zhong W, Xu C, Huang X, Wang HW, Ma Y
Cell Res 30 436-445 (2020)
PMID: 32047270
Related structures: EMD-30048

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210740 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Pituitary adenylate cyclase-activating polypeptide type I receptor Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 47.32 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P41586 (Residues: 23-438; Coverage: 88%)
Gene name: ADCYAP1R1
Sequence domains:
Pituitary adenylate cyclase-activating polypeptide 38 Chain: B
Molecule details ›
Chain: B
Length: 38 amino acids
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18509 (Residues: 132-169; Coverage: 25%)
Gene name: ADCYAP1
Sequence domains: Peptide hormone
Nanobody 35 Chain: C
Molecule details ›
Chain: C
Length: 134 amino acids
Theoretical weight: 14.71 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: E
Molecule details ›
Chain: E
Length: 341 amino acids
Theoretical weight: 37.47 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: F
Molecule details ›
Chain: F
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Insect BA phytoplasma
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-30048
Expression systems:
  • Insect BA phytoplasma
  • Not provided
  • Escherichia coli

Quick links

Citations

6 review citations

Novel Analgesics with Peripheral Targets.
Ciotu et al. (2020)
5 more

9 mentions without citation

Targeting VIP and PACAP Receptor Signaling: New Insights into Designing Drugs for the PACAP Subfamily of Receptors.
Lu et al. (2022)
8 more