› 6m9t

X-ray diffraction
2.5Å resolution

Crystal structure of EP3 receptor bound to misoprostol-FA

Released:
DOI: 10.2210/pdb6m9t/pdb
PDB entry 6m9t coloured by chain, front view.
PDB entry 6m9t coloured by chain, side view.
PDB entry 6m9t coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of misoprostol bound to the labor inducer prostaglandin E2 receptor.
Audet M, White KL, Breton B, Zarzycka B, Han GW, Lu Y, Gati C, Batyuk A, Popov P, Velasquez J, Manahan D, Hu H, Weierstall U, Liu W, Shui W, Katritch V, Cherezov V, Hanson MA, Stevens RC
Nat Chem Biol 15 11-17 (2019)
PMID: 30510194

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Prostaglandin E2 receptor EP3 subtype Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 60.02 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43115 (Residues: 2-259, 273-353; Coverage: 87%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, PTGER3
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand J9P 1 x J9P
Ligand SO4 4 x SO4
Ligand OLC 1 x OLC
Ligand OLA 4 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2
Unit cell:
a: 120.8Å b: 54.85Å c: 96.8Å
α: 90° β: 95.83° γ: 90°
R-values:
R R work R free
0.202 0.2 0.242
Expression system: Spodoptera frugiperda

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Citations

1 review citation

An outlook on using serial femtosecond crystallography in drug discovery.
Mishin et al. (2019)
12 other articles

11 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
10 more