› 6mac

X-ray diffraction
2.34Å resolution

Ternary structure of GDF11 bound to ActRIIB-ECD and Alk5-ECD

Released:
DOI: 10.2210/pdb6mac/pdb
PDB entry 6mac coloured by chain, front view.
PDB entry 6mac coloured by chain, side view.
PDB entry 6mac coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of an activin class ternary receptor complex reveals a third paradigm for receptor specificity.
Goebel EJ, Corpina RA, Hinck CS, Czepnik M, Castonguay R, Grenha R, Boisvert A, Miklossy G, Fullerton PT, Matzuk MM, Idone VJ, Economides AN, Kumar R, Hinck AP, Thompson TB
Proc Natl Acad Sci U S A 116 15505-15513 (2019)
PMID: 31315975

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-131910 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Growth/differentiation factor 11 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.36 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: O95390 (Residues: 300-407; Coverage: 28%)
Gene names: BMP11, GDF11
Sequence domains: Transforming growth factor beta like domain
Activin receptor type-2B Chain: C
Molecule details ›
Chain: C
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P38445 (Residues: 26-120; Coverage: 19%)
Gene names: Actriib, Acvr2b
Sequence domains: Activin types I and II receptor domain
TGF-beta receptor type-1 Chain: K
Molecule details ›
Chain: K
Length: 80 amino acids
Theoretical weight: 8.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36897 (Residues: 33-112; Coverage: 17%)
Gene names: ALK5, SKR4, TGFBR1
Sequence domains: Activin types I and II receptor domain

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3212
Unit cell:
a: 116.624Å b: 116.624Å c: 56.625Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.222 0.267
Expression systems:
  • Cricetulus griseus
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

9 review citations

Receptor binding competition: A paradigm for regulating TGF-β family action.
Martinez-Hackert et al. (2021)
8 more

6 mentions without citation

Structural perspective of BMP ligands and signaling.
Gipson et al. (2020)
5 more