› 6mbj

X-ray diffraction
1.78Å resolution

SETD3, a Histidine Methyltransferase, in Complex with an Actin Peptide and SAH, P21 Crystal Form

Released:
DOI: 10.2210/pdb6mbj/pdb
PDB entry 6mbj coloured by chain, front view.
PDB entry 6mbj coloured by chain, side view.
PDB entry 6mbj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SETD3 is an actin histidine methyltransferase that prevents primary dystocia.
Wilkinson AW, Diep J, Dai S, Liu S, Ooi YS, Song D, Li TM, Horton JR, Zhang X, Liu C, Trivedi DV, Ruppel KM, Vilches-Moure JG, Casey KM, Mak J, Cowan T, Elias JE, Nagamine CM, Spudich JA, Cheng X, Carette JE, Gozani O
Nature 565 372-376 (2019)
PMID: 30626964

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1, N-terminally processed Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P60709 (Residues: 66-80; Coverage: 4%)
Gene name: ACTB
Actin-histidine N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 599 amino acids
Theoretical weight: 67.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86TU7 (Residues: 1-594; Coverage: 100%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
3 bound ligands:
Ligand EDO 35 x EDO
Ligand GOL 3 x GOL
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 60.351Å b: 176.168Å c: 66.577Å
α: 90° β: 92.86° γ: 90°
R-values:
R R work R free
0.2 0.199 0.223
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

16 review citations

Histone lysine methyltransferases in biology and disease.
Husmann et al. (2019)
15 more

4 mentions without citation

SETD3 is an actin histidine methyltransferase that prevents primary dystocia.
Wilkinson et al. (2019)
3 more