6mec

Electron Microscopy
3.6Å resolution

Structure of a group II intron retroelement after DNA integration

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-113221 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (3 distinct):
Reverse transcriptase domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 562 amino acids
Theoretical weight: 65.07 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DMK2 (Residues: 1-562; Coverage: 100%)
Gene name: tll0114
Sequence domains:
Sense Target DNA Chain: B
Molecule details ›
Chain: B
Length: 45 nucleotides
Theoretical weight: 14.03 KDa
Source organism: Thermosynechococcus vestitus
Expression system: Not provided
T.el4h RNA Chain: A
Molecule details ›
Chain: A
Length: 867 nucleotides
Theoretical weight: 280.85 KDa
Source organism: Thermosynechococcus vestitus
Expression system: Escherichia coli
Sequence domains:
  • Group II catalytic intron D1-D4-7
  • Group II catalytic intron
  • Ligands and Environments

    2 bound ligands:
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.6Å
    Relevant EMDB volumes: EMD-9106
    Expression systems:
    • Escherichia coli
    • Not provided