› 6mjl

X-ray diffraction
2.5Å resolution

Crystal structure of ChREBP NLS peptide bound to importin alpha.

Released:
DOI: 10.2210/pdb6mjl/pdb
PDB entry 6mjl coloured by chain, front view.
PDB entry 6mjl coloured by chain, side view.
PDB entry 6mjl coloured by chain, top view.
Source organisms:
Primary publication:
The structure of importin α and the nuclear localization peptide of ChREBP, and small compound inhibitors of ChREBP-importin α interactions.
Jung H, Takeshima T, Nakagawa T, MacMillan KS, Wynn RM, Wang H, Sakiyama H, Wei S, Li Y, Bruick RK, Posner BA, De Brabander JK, Uyeda K
Biochem J 477 3253-3269 (2020)
PMID: 32776146

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 46.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 72-497; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
Carbohydrate-responsive element-binding protein Chain: A
Molecule details ›
Chain: A
Length: 9 amino acids
Theoretical weight: 1.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NP71 (Residues: 168-176; Coverage: 1%)
Gene names: BHLHD14, MIO, MLXIPL, WBSCR14

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 77.434Å b: 89.691Å c: 96.332Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Nuclear-Import Receptors Counter Deleterious Phase Transitions in Neurodegenerative Disease.
Odeh et al. (2022)
1 more

1 mention without citation

The structure of importin α and the nuclear localization peptide of ChREBP, and small compound inhibitors of ChREBP-importin α interactions.
Jung et al. (2020)