› 6mks

Electron Microscopy
3.4Å resolution

Cryo-EM structure of NLRC4-CARD filament

Released:
DOI: 10.2210/pdb6mks/pdb
PDB entry 6mks coloured by chain, front view.
PDB entry 6mks coloured by chain, side view.
PDB entry 6mks coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the NLRC4CARD filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly.
Matyszewski M, Zheng W, Lueck J, Antiochos B, Egelman EH, Sohn J
J Biol Chem 293 20240-20248 (2018)
PMID: 30385506
Related structures: EMD-9137

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 31-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; NLR family CARD domain-containing protein 4 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e
Length: 340 amino acids
Theoretical weight: 38.67 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NPP4 (Residues: 1-96; Coverage: 9%)
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
Gene names: CARD12, CLAN, CLAN1, GFP, IPAF, NLRC4, UNQ6189/PRO20215
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9137
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure, Activation and Regulation of NLRP3 and AIM2 Inflammasomes.
Sharma et al. (2021)
7 more

1 mention without citation

Cryo-EM structure of the NLRC4CARD filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly.
Matyszewski et al. (2018)