› 6mlq

Electron Microscopy
4.2Å resolution

Cryo-EM structure of microtubule-bound Kif7 in the ADP state

Released:
DOI: 10.2210/pdb6mlq/pdb
PDB entry 6mlq coloured by chain, front view.
PDB entry 6mlq coloured by chain, side view.
PDB entry 6mlq coloured by chain, top view.
Source organisms:
Primary publication:
Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary Kinesin Kif7.
Jiang S, Mani N, Wilson-Kubalek EM, Ku PI, Milligan RA, Subramanian R
Dev Cell 49 711-730.e8 (2019)
PMID: 31031197
Related structures: EMD-9140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135904 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1A chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.12 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02550 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1A
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Kinesin-like protein KIF7 Chain: C
Molecule details ›
Chain: C
Length: 399 amino acids
Theoretical weight: 43.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2M1P5 (Residues: 1-398; Coverage: 30%)
Gene names: KIF7, UNQ340/PRO539
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-9140
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Developmental and regenerative paradigms of cilia regulated hedgehog signaling.
Kopinke et al. (2021)
1 more

2 mentions without citation

Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary Kinesin Kif7.
Jiang et al. (2019)
1 more