› 6mol

X-ray diffraction
3.16Å resolution

Monoextended DARPin M_R12 complex with EpoR

Released:
DOI: 10.2210/pdb6mol/pdb
PDB entry 6mol coloured by chain, front view.
PDB entry 6mol coloured by chain, side view.
PDB entry 6mol coloured by chain, top view.
Source organisms:
Primary publication:
Topological control of cytokine receptor signaling induces differential effects in hematopoiesis.
Mohan K, Ueda G, Kim AR, Jude KM, Fallas JA, Guo Y, Hafer M, Miao Y, Saxton RA, Piehler J, Sankaran VG, Baker D, Garcia KC
Science 364 (2019)
PMID: 31123111

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148536 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Monoextended DARPin R12 (M_R12) Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 48.22 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Erythropoietin receptor Chains: B, C
Molecule details ›
Chains: B, C
Length: 229 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P19235 (Residues: 32-249; Coverage: 45%)
Gene name: EPOR
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P63
Unit cell:
a: 168.109Å b: 168.109Å c: 78.029Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.219 0.26
Expression systems:
  • Escherichia coli
  • Trichoplusia ni

Quick links

Citations

12 review citations

Recent advances in de novo protein design: Principles, methods, and applications.
Pan et al. (2021)
11 more

4 mentions without citation

Topological control of cytokine receptor signaling induces differential effects in hematopoiesis.
Mohan et al. (2019)
3 more