› 6mun

Solution NMR

Structure of hRpn10 bound to UBQLN2 UBL

Released:
DOI: 10.2210/pdb6mun/pdb
PDB entry 6mun coloured by chain, ensemble of 10 models, front view.
PDB entry 6mun coloured by chain, ensemble of 10 models, side view.
PDB entry 6mun coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome.
Chen X, Ebelle DL, Wright BJ, Sridharan V, Hooper E, Walters KJ
J Mol Biol 431 939-955 (2019)
PMID: 30664872

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157154 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome non-ATPase regulatory subunit 4 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 11.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55036 (Residues: 196-306; Coverage: 29%)
Gene names: MCB1, PSMD4
Sequence domains: Ubiquitin interaction motif
Ubiquilin-2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 78 amino acids
Theoretical weight: 8.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHD9 (Residues: 26-103; Coverage: 13%)
Gene names: HRIHFB2157, N4BP4, PLIC2, UBQLN2
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Mechanisms of substrate recognition by the 26S proteasome.
Davis et al. (2021)
9 more

2 mentions without citation

Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome.
Chen et al. (2019)
1 more