› 6mw7

X-ray diffraction
2.19Å resolution

Crystal structure of ATPase module of SMCHD1 bound to ATP

Released:
DOI: 10.2210/pdb6mw7/pdb
PDB entry 6mw7 coloured by chain, front view.
PDB entry 6mw7 coloured by chain, side view.
PDB entry 6mw7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A ubiquitin-like domain is required for stabilizing the N-terminal ATPase module of human SMCHD1.
Pedersen LC, Inoue K, Kim S, Perera L, Shaw ND
Commun Biol 2 255 (2019)
PMID: 31312724

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107996 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Structural maintenance of chromosomes flexible hinge domain-containing protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 562 amino acids
Theoretical weight: 65.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6NHR9 (Residues: 24-580; Coverage: 28%)
Gene names: KIAA0650, SMCHD1
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Ligands and Environments

4 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 4 x MG
Ligand SO4 1 x SO4
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 108.756Å b: 148.012Å c: 191.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Consequences of epigenetic derepression in facioscapulohumeral muscular dystrophy.
Greco et al. (2020)
2 other articles

2 mentions without citation

Relating SMCHD1 structure to its function in epigenetic silencing.
Gurzau et al. (2020)
1 more