› 6mye

X-ray diffraction
1.1Å resolution

Crystal structure of human Scribble PDZ1 domain in complex with internal PDZ binding motif of Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF)

Released:
DOI: 10.2210/pdb6mye/pdb
PDB entry 6mye coloured by chain, front view.
PDB entry 6mye coloured by chain, side view.
PDB entry 6mye coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.
Awadia S, Huq F, Arnold TR, Goicoechea SM, Sun YJ, Hou T, Kreider-Letterman G, Massimi P, Banks L, Fuentes EJ, Miller AL, Garcia-Mata R
J Cell Biol 218 2699-2725 (2019)
PMID: 31248911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171948 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein scribble homolog Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 9.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14160 (Residues: 725-815; Coverage: 6%)
Gene names: CRIB1, KIAA0147, LAP4, SCRB1, SCRIB, VARTUL
Sequence domains: PDZ domain
Rho guanine nucleotide exchange factor 26 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96DR7 (Residues: 42-52; Coverage: 1%)
Gene names: ARHGEF26, HMFN1864, SGEF

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P43212
Unit cell:
a: 67.89Å b: 67.89Å c: 44.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.204
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Ruffles and spikes: Control of tight junction morphology and permeability by claudins.
Lynn et al. (2020)
3 more

1 mention without citation

Integrated analysis of Shank1 PDZ interactions with C-terminal and internal binding motifs.
Ali et al. (2021)