› 6mzm

Electron Microscopy
7.5Å resolution

Human TFIID bound to promoter DNA and TFIIA

Released:
DOI: 10.2210/pdb6mzm/pdb
PDB entry 6mzm coloured by chain, front view.
PDB entry 6mzm coloured by chain, side view.
PDB entry 6mzm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
Patel AB, Louder RK, Greber BJ, Grünberg S, Luo J, Fang J, Liu Y, Ranish J, Hahn S, Nogales E
Science 362 (2018)
PMID: 30442764
Related structures: EMD-9306

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120309 (preferred)
Entry contents:
15 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (17 distinct):
Transcription initiation factor TFIID subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 57.19 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P21675 (Residues: 609-1104; Coverage: 26%)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains: Protein of unknown function (DUF3591)
Transcription initiation factor TFIID subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 1199 amino acids
Theoretical weight: 137.16 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6P1X5 (Residues: 1-1199; Coverage: 100%)
Gene names: CIF150, TAF2, TAF2B
Transcription initiation factor TFIID subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 1025 amino acids
Theoretical weight: 104.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00268 (Residues: 61-1085; Coverage: 95%)
Gene names: TAF2C, TAF2C1, TAF4, TAF4A, TAFII130, TAFII135
Sequence domains:
Transcription initiation factor TFIID subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 800 amino acids
Theoretical weight: 85.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15542 (Residues: 1-800; Coverage: 100%)
Gene names: TAF2D, TAF5
Sequence domains:
Transcription initiation factor TFIID subunit 6 Chain: H
Molecule details ›
Chain: H
Length: 677 amino acids
Theoretical weight: 72.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49848 (Residues: 1-677; Coverage: 100%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains:
Transcription initiation factor TFIID subunit 6 Chain: I
Molecule details ›
Chain: I
Length: 677 amino acids
Theoretical weight: 72.37 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49848 (Residues: 1-677; Coverage: 100%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains:
Transcription initiation factor TFIID subunit 7 Chain: J
Molecule details ›
Chain: J
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15545 (Residues: 1-349; Coverage: 100%)
Gene names: TAF2F, TAF7, TAFII55
Sequence domains: TAFII55 protein conserved region
Transcription initiation factor TFIID subunit 8 Chain: K
Molecule details ›
Chain: K
Length: 310 amino acids
Theoretical weight: 32.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q7Z7C8 (Residues: 1-310; Coverage: 100%)
Gene names: TAF8, TAFII43, TBN
Sequence domains:
Transcription initiation factor TFIID subunit 9 Chain: L
Molecule details ›
Chain: L
Length: 264 amino acids
Theoretical weight: 28.83 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q16594 (Residues: 1-264; Coverage: 100%)
Gene names: TAF2G, TAF9, TAFII31
Sequence domains: Transcription initiation factor IID, 31kD subunit
Transcription initiation factor TFIID subunit 10 Chain: O
Molecule details ›
Chain: O
Length: 218 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q12962 (Residues: 1-218; Coverage: 100%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30
Sequence domains: Transcription initiation factor TFIID 23-30kDa subunit
Transcription initiation factor TFIID subunit 12 Chain: R
Molecule details ›
Chain: R
Length: 161 amino acids
Theoretical weight: 17.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q16514 (Residues: 1-161; Coverage: 100%)
Gene names: TAF12, TAF15, TAF2J, TAFII20
Sequence domains: Transcription initiation factor TFIID subunit A
TATA-box-binding protein Chain: T
Molecule details ›
Chain: T
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20226 (Residues: 1-339; Coverage: 100%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Transcription initiation factor IIA subunit 1 Chain: W
Molecule details ›
Chain: W
Length: 90 amino acids
Theoretical weight: 10.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52655 (Residues: 9-376; Coverage: 24%)
Gene names: GTF2A1, TF2A1
Sequence domains: Transcription factor IIA, alpha/beta subunit
Transcription initiation factor IIA subunit 2 Chain: X
Molecule details ›
Chain: X
Length: 97 amino acids
Theoretical weight: 11.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52657 (Residues: 3-99; Coverage: 89%)
Gene names: GTF2A2, TF2A2
Sequence domains:
Unk Chain: Z
Molecule details ›
Chain: Z
Length: 238 amino acids
Theoretical weight: 20.27 KDa
Source organism: Homo sapiens
SCP DNA (80-MER) Chain: U
Molecule details ›
Chain: U
Length: 80 nucleotides
Theoretical weight: 24.5 KDa
SCP DNA (80-MER) Chain: V
Molecule details ›
Chain: V
Length: 80 nucleotides
Theoretical weight: 24.85 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.5Å
Relevant EMDB volumes: EMD-9306
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Challenges and opportunities in cryo-EM single-particle analysis.
Lyumkis D. (2019)
20 more

5 mentions without citation

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
4 more