› 6n1i

Electron Microscopy
3.58Å resolution

Cryo-EM structure of NLRC4-CARD filament

Released:
DOI: 10.2210/pdb6n1i/pdb
PDB entry 6n1i coloured by chain, front view.
PDB entry 6n1i coloured by chain, side view.
PDB entry 6n1i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1.
Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H
Proc Natl Acad Sci U S A 115 10845-10852 (2018)
PMID: 30279182
Related structures: EMD-8903

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NLR family CARD domain-containing protein 4 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 85 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NPP4 (Residues: 1-85; Coverage: 8%)
Gene names: CARD12, CLAN, CLAN1, IPAF, NLRC4, UNQ6189/PRO20215
Sequence domains: Caspase recruitment domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-8903
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Inflammasome activation and regulation: toward a better understanding of complex mechanisms.
Zheng et al. (2020)
30 more

5 mentions without citation

Assembly of platforms for signal transduction in the new era: dimerization, helical filament assembly, and beyond.
Ha et al. (2020)
4 more