› 6n1r

Electron Microscopy
4Å resolution

Tetrahedral oligomeric complex of GyrA N-terminal fragment, solved by cryoEM in tetrahedral symmetry

Released:
DOI: 10.2210/pdb6n1r/pdb
PDB entry 6n1r coloured by chain, front view.
PDB entry 6n1r coloured by chain, side view.
PDB entry 6n1r coloured by chain, top view.
Source organism: Streptococcus pneumoniae G54
Primary publication:
CryoEM structures of open dimers of gyrase A in complex with DNA illuminate mechanism of strand passage.
Soczek KM, Grant T, Rosenthal PB, Mondragón A
Elife 7 (2018)
PMID: 30457554
Related structures: EMD-9318

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit A Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 511 amino acids
Theoretical weight: 57.98 KDa
Source organism: Streptococcus pneumoniae G54
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DPM2 (Residues: 1-487; Coverage: 59%)
Gene names: gyrA, spr1099
Sequence domains: DNA gyrase/topoisomerase IV, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-9318
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure-Function Analysis Reveals the Singularity of Plant Mitochondrial DNA Replication Components: A Mosaic and Redundant System.
Brieba LG. (2019)
3 more