› 6n3k

X-ray diffraction
2.2Å resolution

Crystal structure of an epoxide hydrolase from Trichoderma reesei in complex with inhibitor 1

Released:
DOI: 10.2210/pdb6n3k/pdb
PDB entry 6n3k coloured by chain, front view.
PDB entry 6n3k coloured by chain, side view.
PDB entry 6n3k coloured by chain, top view.
Source organism: Trichoderma reesei QM9414
Primary publication:
The molecular structure of an epoxide hydrolase from Trichoderma reesei in complex with urea or amide-based inhibitors.
de Oliveira GS, Adriani PP, Ribeiro JA, Morisseau C, Hammock BD, Dias MVB, Chambergo FS
Int J Biol Macromol 129 653-658 (2019)
PMID: 30771398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.53 KDa
Source organism: Trichoderma reesei QM9414
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: G0R7E2 (Residues: 1-335; Coverage: 98%)
Gene name: TRIREDRAFT_53220
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand K9V 1 x K9V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Unit cell:
a: 50.073Å b: 77.276Å c: 87.103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.248
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 citations in other articles

A Genomic and Transcriptomic Study on the DDT-Resistant Trichoderma hamatum FBL 587: First Genetic Data into Mycoremediation Strategies for DDT-Polluted Sites.
Davolos et al. (2021)