› 6n51

Electron Microscopy
4Å resolution

Metabotropic Glutamate Receptor 5 bound to L-quisqualate and Nb43

Released:
DOI: 10.2210/pdb6n51/pdb
PDB entry 6n51 coloured by chain, front view.
PDB entry 6n51 coloured by chain, side view.
PDB entry 6n51 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the activation of metabotropic glutamate receptors.
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laeremans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature 566 79-84 (2019)
PMID: 30675062
Related structures: EMD-0345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211199 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metabotropic glutamate receptor 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 804 amino acids
Theoretical weight: 90.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41594 (Residues: 23-826; Coverage: 67%)
Gene names: GPRC1E, GRM5, MGLUR5
Sequence domains:
Nanobody 43 Chains: C, D
Molecule details ›
Chains: C, D
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand QUS 2 x QUS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-0345
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Covalent allosteric modulation: An emerging strategy for GPCRs drug discovery.
Bian et al. (2020)
27 more

29 mentions without citation

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
28 more