› 6ncm

X-ray diffraction
2.7Å resolution

Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead-like (FHL) DNA sequence

Released:
DOI: 10.2210/pdb6ncm/pdb
PDB entry 6ncm coloured by chain, front view.
PDB entry 6ncm coloured by chain, side view.
PDB entry 6ncm coloured by chain, top view.
Source organisms:
Primary publication:
Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes.
Rogers JM, Waters CT, Seegar TCM, Jarrett SM, Hallworth AN, Blacklow SC, Bulyk ML
Mol Cell 74 245-253.e6 (2019)
PMID: 30826165

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120331 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein N3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 11.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00409 (Residues: 112-210; Coverage: 20%)
Gene names: C14orf116, CHES1, FOXN3
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*TP*AP*GP*CP*GP*TP*CP*TP*TP*AP*GP*CP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*TP*GP*CP*TP*AP*AP*GP*AP*CP*GP*CP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 42.54Å b: 72.31Å c: 102.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Recent Advances in Understanding FOXN3 in Breast Cancer, and Other Malignancies.
Kong et al. (2019)
2 more

2 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
1 more