› 6ne0

Electron Microscopy
3.4Å resolution

Structure of double-stranded target DNA engaged Csy complex from Pseudomonas aeruginosa (PA-14)

Released:
DOI: 10.2210/pdb6ne0/pdb
PDB entry 6ne0 coloured by chain, front view.
PDB entry 6ne0 coloured by chain, side view.
PDB entry 6ne0 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry.
Rollins MF, Chowdhury S, Carter J, Golden SM, Miettinen HM, Santiago-Frangos A, Faith D, Lawrence CM, Lander GC, Wiedenheft B
Mol Cell 74 132-142.e5 (2019)
PMID: 30872121
Related structures: EMD-9191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-120332 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 342 amino acids
Theoretical weight: 37.58 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Molecule details ›
Chain: L
Length: 189 amino acids
Theoretical weight: 21.68 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
CRISPR target DNA (44-MER) Chain: N
Molecule details ›
Chain: N
Length: 44 nucleotides
Theoretical weight: 13.58 KDa
Non-complementary R-loop DNA strand Chain: O
Molecule details ›
Chain: O
Length: 34 nucleotides
Theoretical weight: 10.48 KDa
CRISPR RNA (60-MER) Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.27 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems.
Davidson et al. (2020)
6 more

9 mentions without citation

Structures and Strategies of Anti-CRISPR-Mediated Immune Suppression.
Wiegand et al. (2020)
8 more