6ne3

Electron Microscopy
3.9Å resolution

Cryo-EM structure of singly-bound SNF2h-nucleosome complex with SNF2h bound at SHL-2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-120333 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 130 amino acids
Theoretical weight: 14.09 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H2B 1.1 Chain: D
Molecule details ›
Chain: D
Length: 99 amino acids
Theoretical weight: 11.18 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 28-126; Coverage: 79%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H2B 1.1 Chain: H
Molecule details ›
Chain: H
Length: 93 amino acids
Theoretical weight: 10.35 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 33-125; Coverage: 74%)
Sequence domains: Core histone H2A/H2B/H3/H4
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 Chain: W
Molecule details ›
Chain: W
Length: 467 amino acids
Theoretical weight: 54.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60264 (Residues: 166-634; Coverage: 44%)
Gene names: SMARCA5, SNF2H, WCRF135
Sequence domains:
DNA (156-MER) Chain: J
Molecule details ›
Chain: J
Length: 156 nucleotides
Theoretical weight: 47.94 KDa
DNA (156-MER) Chain: I
Molecule details ›
Chain: I
Length: 156 nucleotides
Theoretical weight: 48.37 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-9356
Expression system: Escherichia coli