› 6nf0

X-ray diffraction
2.7Å resolution

Nocturnin with bound NADPH substrate

Released:
DOI: 10.2210/pdb6nf0/pdb
PDB entry 6nf0 coloured by chain, front view.
PDB entry 6nf0 coloured by chain, side view.
PDB entry 6nf0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The metabolites NADP+ and NADPH are the targets of the circadian protein Nocturnin (Curled).
Estrella MA, Du J, Chen L, Rath S, Prangley E, Chitrakar A, Aoki T, Schedl P, Rabinowitz J, Korennykh A
Nat Commun 10 2367 (2019)
PMID: 31147539

Function and Biology Details

Reaction catalysed: 
NADP(+) + H(2)O = NAD(+) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nocturnin Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 35.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UK39 (Residues: 122-431; Coverage: 72%)
Gene names: CCR4, CCRN4L, NOC, NOCT
Sequence domains: Endonuclease/Exonuclease/phosphatase family

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand NDP 1 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P41212
Unit cell:
a: 62.888Å b: 62.888Å c: 153.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.264
Expression system: Escherichia coli

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Citations

8 review citations

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Cheung et al. (2022)
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